2UZI

Crystal structure of HRAS(G12V) - anti-RAS Fv complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2005-11-11
Detector	ADSC CCD
Spacegroup name	P 21 21 2
Unit cell lengths	75.528, 84.632, 62.592
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.890 - 2.000
R-factor	0.198
Rwork	0.194
R-free	0.27100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1agp 1a2y
RMSD bond length	0.019
RMSD bond angle	1.812
Data reduction software	MOSFLM
Data scaling software	TRUNCATE
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.320	2.020
High resolution limit [Å]	2.000	2.000
Rmerge	0.100	0.360
Number of reflections	27214
<I/σ(I)>	10.42	2.67
Completeness [%]	98.2	97.2
Redundancy	3.38	3.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				PROTEIN WAS CRYSTALLIZED FROM 17-18 % PEG3350, 400 MM ZINC ACETATE, 100 MM SODIUM CACODYLATE, PH 5.8, 0.03 % DICHLOROMETHANE