2SHP
TYROSINE PHOSPHATASE SHP-2
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-02 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.900, 214.500, 55.700 |
| Unit cell angles | 90.00, 96.30, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT, NCS AVERAGING |
| Starting model (for MR) | PTP1B AND N-TERMINAL SH2 DOMAINS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.546 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.100 | |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.062 | 0.295 |
| Total number of observations | 276084 * | |
| Number of reflections | 71634 | |
| Completeness [%] | 93.9 | 82.5 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | dithiothreitol | 20 (mM) | |
| 5 | 1 | drop | trimethyllead acetate | 5 (mM) | |
| 6 | 1 | drop | CTAB | 1.2 (mM) | |
| 7 | 1 | reservoir | PEG4000 | 13 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 9 | 1 | reservoir | dithiothreitol | 20 (mM) |
