2RH1

High resolution crystal structure of human B2-adrenergic G protein-coupled receptor.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	78
Detector technology	CCD
Collection date	2007-06-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03321
Spacegroup name	C 1 2 1
Unit cell lengths	106.318, 169.240, 40.154
Unit cell angles	90.00, 105.62, 90.00

Refinement procedure

Resolution	19.950 - 2.400
R-factor	0.198
Rwork	0.196
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 1U19 2lzm
RMSD bond length	0.013
RMSD bond angle	1.500
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.500
High resolution limit [Å]	2.400	10.000	2.400
Rmerge	0.127	0.066	0.678
Number of reflections	26506	333	3060
<I/σ(I)>	9.62	23.2	2.2
Completeness [%]	99.2	87.4	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC MESOPHASE	6.75	293	30-35% v/v PEG 400, 0.1-0.2 M Na2SO4, 0.1 M Bis-tris propane pH 6.5-7.0, 5-7% 1,4-Butanediol, 8-10% Cholesterol, 52-50% Monoolein, pH 6.75, LIPIDIC MESOPHASE, temperature 293K
1	LIPIDIC MESOPHASE	6.75	293	30-35% v/v PEG 400, 0.1-0.2 M Na2SO4, 0.1 M Bis-tris propane pH 6.5-7.0, 5-7% 1,4-Butanediol, 8-10% Cholesterol, 52-50% Monoolein, pH 6.75, LIPIDIC MESOPHASE, temperature 293K