2RD7
Human Complement Membrane Attack Proteins Share a Common Fold with Bacterial Cytolysins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9565 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 96.553, 126.087, 51.887 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.000 - 2.150 |
R-factor | 0.20827 |
Rwork | 0.206 |
R-free | 0.26086 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Lower resolution model solved by SAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.630 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.048 | 0.400 |
Number of reflections | 32462 | |
<I/σ(I)> | 33.4 | 2.6 |
Completeness [%] | 93.2 | 84.2 |
Redundancy | 4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 6% PEG 20,000, 0.1 M citric acid, 0.025 M Mg(NO3)2, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |