2R7G

Structure of the retinoblastoma protein pocket domain in complex with adenovirus E1A CR1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Detector technology	CCD
Collection date	2006-07-06
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.980
Spacegroup name	P 1
Unit cell lengths	57.713, 57.706, 92.268
Unit cell angles	94.34, 92.69, 105.79

Refinement procedure

Resolution	40.820 - 1.671
R-factor	0.19759
Rwork	0.195
R-free	0.21823
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.256
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.671	3.600	1.671
Rmerge	0.035	0.021	0.392
Number of reflections	121240
<I/σ(I)>	14.6
Completeness [%]	92.0	98.4	56.7
Redundancy	2.5	2.6	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6	293	15mM magnesium acetate tetrahydrate, 50mM sodium cacodylate trihydrate, pH 6.0 and 1.7M ammonium sulfate, VAPOR DIFFUSION, temperature 293K