2QK4
Human glycinamide ribonucleotide synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-25 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.93300 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.280, 79.810, 113.870 |
Unit cell angles | 90.00, 104.07, 90.00 |
Refinement procedure
Resolution | 19.980 - 2.450 |
R-factor | 0.17386 |
Rwork | 0.171 |
R-free | 0.22107 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1gso |
RMSD bond length | 0.014 |
RMSD bond angle | 1.546 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.500 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.049 | 0.341 |
Number of reflections | 44786 | |
<I/σ(I)> | 22.15 | 3.94 |
Completeness [%] | 99.2 | 91.7 |
Redundancy | 5.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 298 | 16% PEG 3350, 0.3M LiSO4, 100 mM Bis-Tris pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |