2QHO
Crystal structure of the UBA domain from EDD ubiquitin ligase in complex with ubiquitin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.08090 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.849, 59.333, 246.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.530 - 1.850 |
| R-factor | 0.20922 |
| Rwork | 0.207 |
| R-free | 0.25807 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq 2ooa |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.051 | 0.292 |
| Number of reflections | 38635 | |
| <I/σ(I)> | 29.5 | 7.1 |
| Completeness [%] | 92.9 | 81 |
| Redundancy | 7 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M citric acid, 20% PEG 6000, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
