2QG4
Crystal structure of human UDP-glucose dehydrogenase product complex with UDP-glucuronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.033 |
| Spacegroup name | H 3 |
| Unit cell lengths | 193.868, 193.868, 352.223 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 59.660 - 2.100 |
| R-factor | 0.17832 |
| Rwork | 0.176 |
| R-free | 0.22496 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q3e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.238 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.139 | |
| Number of reflections | 288088 | |
| <I/σ(I)> | 7.6 | 1.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 20% PEG 3350, 10% Ethylene glycol, 0.2 M NaBr, 0.1 M Bis-tris-propane, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
