2Q8E
Specificity and Mechanism of JMJD2A, a Trimethyllysine-Specific Histone Demethylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2007-02-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 100.294, 148.964, 56.810 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.970 - 2.050 |
| R-factor | 0.21501 |
| Rwork | 0.213 |
| R-free | 0.25966 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gp5 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.561 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.600 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.056 | 0.463 |
| Number of reflections | 54092 | |
| <I/σ(I)> | 11.4 | 3.1 |
| Redundancy | 5.8 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
