2Q8C

Crystal structure of JMJD2A in ternary complex with an histone H3K9me3 peptide and 2-oxoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	200
Detector technology	CCD
Collection date	2007-01-30
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	100.887, 149.121, 57.056
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.900 - 2.047
R-factor	0.20296
Rwork	0.201
R-free	0.23584
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gp5
RMSD bond length	0.011
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.900	2.120
High resolution limit [Å]	2.040	2.040
Rmerge	0.045	0.506
Number of reflections	54585
<I/σ(I)>	39	2.3
Redundancy	5.8	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1