2OXW
Human MMP-12 complexed with the peptide IAG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 51.888, 60.358, 54.521 |
| Unit cell angles | 90.00, 115.73, 90.00 |
Refinement procedure
| Resolution | 36.960 - 1.150 |
| R-factor | 0.19942 |
| Rwork | 0.197 |
| R-free | 0.22250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y93 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.093 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.080 | 1.210 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.059 | 0.156 |
| Number of reflections | 50850 | |
| <I/σ(I)> | 4.2 | 4.1 |
| Completeness [%] | 94.6 | 91.1 |
| Redundancy | 6.8 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 0.1 M Tris-HCl, 30% PEG6000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
