2OKJ
The X-ray crystal structure of the 67kDa isoform of Glutamic Acid Decarboxylase (GAD67)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.048, 62.739, 101.346 |
| Unit cell angles | 90.00, 106.68, 90.00 |
Refinement procedure
| Resolution | 97.130 - 2.300 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1js3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.130 | |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.045 | 0.194 |
| Number of reflections | 42284 | |
| <I/σ(I)> | 17.1 | 4.4 |
| Completeness [%] | 93.7 | |
| Redundancy | 3.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | 18% PEG8,000, 100 mM MES, pH 6.3, 10 mM 2-mercaptoethanol and 20 mM CaCl2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
