2OAX
Crystal structure of the S810L mutant mineralocorticoid receptor associated with SC9420
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 81 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.979707 |
| Spacegroup name | P 32 |
| Unit cell lengths | 122.198, 122.198, 91.808 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.920 - 2.290 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | monomere A from PDB 1Y9R |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.920 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.121 | 0.623 |
| Number of reflections | 63172 | |
| <I/σ(I)> | 13 | 2.66 |
| Completeness [%] | 91.1 | 94.8 |
| Redundancy | 6.18 | 5.65 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 297 | HEPES 100 mM, pH 6.8, 230 mM NaCl, 25% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
