2O2K
Crystal Structure of the Activation Domain of Human Methionine Synthase Isoform/Mutant D963E/K1071N
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2005-12-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.854, 90.051, 123.006 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.830 - 1.600 |
R-factor | 0.2113 |
Rwork | 0.209 |
R-free | 0.24782 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1msk |
RMSD bond length | 0.006 |
RMSD bond angle | 1.038 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.660 |
High resolution limit [Å] | 1.600 | 1.600 |
Number of reflections | 106145 | |
<I/σ(I)> | 20.1 | 3.2 |
Completeness [%] | 96.3 | 96.5 |
Redundancy | 5.3 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292 | 0.1M Tris/HCl, 0.1-0.4M sodium acetate, 10-12% PEG 8000, 10-12% PEG 1000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 292.0K |