2JHT
CRYSTAL STRUCTURE OF RHOGDI K135T,K138T,K141T MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-19 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 190.832, 34.022, 103.940 |
| Unit cell angles | 90.00, 115.59, 90.00 |
Refinement procedure
| Resolution | 93.660 - 1.880 |
| R-factor | 0.223 |
| Rwork | 0.222 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.130 | 0.260 |
| Number of reflections | 44864 | |
| <I/σ(I)> | 14.6 | 3.6 |
| Completeness [%] | 91.5 | 64.4 |
| Redundancy | 4.8 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 35% PEG 4000 0.2 M LITHIUM SULFATE 0.1M TRIS PH 8.5 |
