2JB6
Fab fragment in complex with small molecule hapten, crystal form-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.028, 77.028, 379.128 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.400 - 2.850 |
| R-factor | 0.231 |
| Rwork | 0.229 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jb5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.283 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.900 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.100 | 0.390 |
| Number of reflections | 27756 | |
| <I/σ(I)> | 15.6 | 3.5 |
| Completeness [%] | 98.7 | 78.8 |
| Redundancy | 5.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 2.6-2.7M AMMONIUM SULPHATE, 5% PEG400, 0.1M SODIUM CITRATE PH 4. ADDITIONAL 10% GLYCEROL AS CRYO BUFFER. |
