2IZV
CRYSTAL STRUCTURE OF SOCS-4 IN COMPLEX WITH ELONGIN-B AND ELONGIN-C AT 2.55A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-16 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 |
| Unit cell lengths | 154.770, 154.770, 67.909 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 77.380 - 2.550 |
| R-factor | 0.175 |
| Rwork | 0.172 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c9w |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.438 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.680 | 2.650 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.130 | 0.360 |
| Number of reflections | 19724 | |
| <I/σ(I)> | 9.14 | 1.98 |
| Completeness [%] | 99.7 | 97.2 |
| Redundancy | 2.71 | 1.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 150 UL SITTING DROP 2M NACL 10% PEK6K |
