2IM1

Crystal structure of poliovirus polymerase complexed with CTP and Mn2+

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	128.942, 128.942, 112.979
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.200 - 2.500
Rwork	0.239
R-free	0.26800
Structure solution method	MAD
Starting model (for MR)	1ra6
RMSD bond length	0.007
RMSD bond angle	1.400
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	36470
<I/σ(I)>	15.5	4.7
Completeness [%]	98.8	98.5
Redundancy	8.31	8.29

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	sodium acetate, cacodylate, DTT, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K