2IK8
Crystal structure of the heterodimeric complex of human RGS16 and activated Gi alpha 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-24 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9198 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.656, 104.312, 124.048 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.600 - 2.710 |
R-factor | 0.23544 |
Rwork | 0.233 |
R-free | 0.29106 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID = 2GTP |
RMSD bond length | 0.008 |
RMSD bond angle | 1.060 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 27900 | |
Completeness [%] | 99.3 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.2M (NH4)2SO4, 0.1M Bis-Tris, pH 5.5, 25% PEG3350 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |