2IFV

Crystal structure of an active site mutant, C473D, of CDC25B phosphatase catalytic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	49.895, 71.189, 73.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.690 - 1.600
R-factor	0.179
Rwork	0.179
R-free	0.19200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ymk
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.600
Number of reflections	34010
<I/σ(I)>	32.8
Completeness [%]	96.0
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	DTT, AMMONIUM SULFATE, TRIS, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K