2IEJ

Human Protein Farnesyltransferase Complexed with Inhibitor Compound STN-48 And FPP Analog at 1.8A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 61
Unit cell lengths	178.253, 178.253, 64.661
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.690 - 1.800
R-factor	0.165
Rwork	0.163
R-free	0.18800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.294
Data reduction software	HKL-2000
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.900
High resolution limit [Å]	1.800	6.000	1.800
Rmerge	0.041	0.018	0.271
Number of reflections	107537
<I/σ(I)>	22.72	59.6	3.7
Completeness [%]	97.6	99.3	88.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2		crystallized via hanging drop against well solution containing 300 mM ammonium acetate pH 5.2, 12% PEG 8K , VAPOR DIFFUSION, HANGING DROP