2HUE
Structure of the H3-H4 chaperone Asf1 bound to histones H3 and H4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 295 |
Detector technology | CCD |
Collection date | 2005-09-30 |
Detector | NOIR-1 |
Wavelength(s) | 1.000, 1.23 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 95.749, 95.749, 110.676 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 11.980 - 1.700 |
Rwork | 0.209 |
R-free | 0.23900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.556 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 12.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 61200 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.1M potassium sulfate, 0.1M Tris-HCl, 14.5% PEG 4K, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |