2HRT
Asymmetric structure of trimeric AcrB from Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-09 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 1 |
Unit cell lengths | 127.330, 134.870, 140.840 |
Unit cell angles | 103.90, 94.64, 90.11 |
Refinement procedure
Resolution | 29.800 - 3.000 |
R-factor | 0.234 |
Rwork | 0.231 |
R-free | 0.27400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1iwg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.059 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.800 | 3.100 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.134 | 0.763 |
Number of reflections | 181133 | |
<I/σ(I)> | 8.4 | 1.9 |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 4.2 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 4.6 | 290 | 5 % polyethylene glycol 400, 16-22 % polyethylene glycol 300, 8-11 % glycerol, 70 mM Nacitrate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 290K, pH 4.60 |