2HKJ
Topoisomerase VI-B bound to radicicol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2005-05-23 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.1157 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 93.879, 111.398, 54.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
R-factor | 0.17025 |
Rwork | 0.167 |
R-free | 0.20877 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1mu5 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.410 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.110 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 39306 | |
<I/σ(I)> | 10.5 | 2.2 |
Completeness [%] | 99.0 | 94.6 |
Redundancy | 3.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 7.5 | 292 | HEPES, Mg(HCO2)2, PEG 3350, NaCl, MgCl2, pH 7.5, microbatch, temperature 292K |