2HC2
Engineered protein tyrosine phosphatase beta catalytic domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Detector technology | CCD |
| Collection date | 2006-02-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.314, 70.775, 49.846 |
| Unit cell angles | 90.00, 92.52, 90.00 |
Refinement procedure
| Resolution | 33.670 - 1.400 |
| R-factor | 0.14571 |
| Rwork | 0.144 |
| R-free | 0.17892 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hc1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.206 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.670 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.028 | 0.210 |
| Number of reflections | 48397 | |
| <I/σ(I)> | 20.2 | 4.87 |
| Completeness [%] | 92.5 | 69.4 |
| Redundancy | 2.6 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.85 | 298 | 21% PEG 8000, 200 mM MgCl2, 1% BME, 0.1% BOG, 1mM DTT, 80 mM Na Citrate, pH 7.85, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
