2H59

Sir2 H116A-deacetylated p53 peptide-3'-o-acetyl ADP ribose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-03
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 41
Unit cell lengths	46.996, 46.996, 257.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.900
R-factor	0.20714
Rwork	0.205
R-free	0.24727
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	: 1yc5
RMSD bond length	0.019
RMSD bond angle	1.935
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900
Number of reflections	42803
<I/σ(I)>	30.6
Completeness [%]	97.9
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2		PEG 8000, Na-Tartrate:K-Phosphate, NaCl, NAD, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 100K