2GZ3
Structure of Aspartate Semialdehyde Dehydrogenase (ASADH) from Streptococcus pneumoniae complexed with NADP and aspartate-semialdehyde
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-15 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.348, 64.439, 96.739 |
| Unit cell angles | 89.98, 81.06, 82.00 |
Refinement procedure
| Resolution | 30.010 - 2.100 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 2GZ1 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.334 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK (9.2SSI) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.580 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.113 | 0.293 |
| Total number of observations | 18514 | |
| Number of reflections | 95735 | |
| <I/σ(I)> | 6.6 | 3.3 |
| Completeness [%] | 92.7 | 89.6 |
| Redundancy | 3.3 | 3.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 50 mM Citrate pH 6.0 200 mM ammonium acetate 20% PEG 8000 10 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
