2G19

Cellular Oxygen Sensing: Crystal Structure of Hypoxia-Inducible Factor Prolyl Hydroxylase (PHD2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	120
Detector technology	CCD
Collection date	2005-12-14
Detector	ADSC QUANTUM 4
Spacegroup name	P 63
Unit cell lengths	110.851, 110.851, 40.379
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.000 - 1.700
Rwork	0.216
R-free	0.25300
Structure solution method	COMBINED MAD AND SIRAS
RMSD bond length	0.007
RMSD bond angle	1.500
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.000	1.730
High resolution limit [Å]	1.700	1.700
Rmerge		0.513
Number of reflections	30804
<I/σ(I)>	32	2
Completeness [%]	97.9	93.6
Redundancy	10	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.4	298	28% polyethylene glycol 8000, 0.2 M ammonium sulfate, 100 mM MES buffer with 10 fold molar excess of inhibitor, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.40
1		6.4	298	28% polyethylene glycol 8000, 0.2 M ammonium sulfate, 100 mM MES buffer with 10 fold molar excess of inhibitor, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K, pH 6.40