2FMO
Ala177Val mutant of E. coli Methylenetetrahydrofolate Reductase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-10-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.153, 128.350, 97.581 |
| Unit cell angles | 90.00, 121.07, 90.00 |
Refinement procedure
| Resolution | 20.010 - 2.250 |
| R-factor | 0.219 |
| Rwork | 0.219 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b5t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.010 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 50832 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 99.7 | 98.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | PEG 4000, LITHIUM SULFATE, SODIUM CACODYLATE, ETHANOL, METHYLPENTANEDIOL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
