2FIM
Structure of the C-terminal domain of Human Tubby-like protein 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-10-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.960, 85.150, 58.790 |
Unit cell angles | 90.00, 92.93, 90.00 |
Refinement procedure
Resolution | 19.890 - 1.900 |
R-factor | 0.201 |
Rwork | 0.199 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.021 |
RMSD bond angle | 1.800 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.061 | 0.239 |
Number of reflections | 43277 | 7645 |
<I/σ(I)> | 15.9 | 5.5 |
Completeness [%] | 99.6 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.2M AS, 25% PEG 4000, 15% glycerol, 50mM 3-(N,N-Dimethyloctylammonio)propanesulfonate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |