2F6J
Crystal structure of PHD finger-linker-bromodomain fragment of human BPTF in the H3(1-15)K4me3 bound state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.3477 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.579, 84.987, 109.385 |
| Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
| Resolution | ? - 2.000 |
| Rwork | 0.198 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.105 | 0.459 |
| Number of reflections | 46877 | |
| <I/σ(I)> | 15 | 4.2 |
| Completeness [%] | 99.5 | 98.7 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 17% PEG 4000, 8.5% iso-Propanol, 0.085M Hepes-Na pH 7.5, 15% Glycerol anhydrous, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
