2ERJ
Crystal structure of the heterotrimeric interleukin-2 receptor in complex with interleukin-2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 1 |
Unit cell lengths | 57.266, 70.545, 129.236 |
Unit cell angles | 83.85, 82.45, 89.72 |
Refinement procedure
Resolution | 129.100 - 3.000 |
R-factor | 0.22254 |
Rwork | 0.220 |
R-free | 0.26288 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB structure 2B5I |
RMSD bond length | 0.009 |
RMSD bond angle | 1.269 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 129.100 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 37249 | |
<I/σ(I)> | 7.8 | 1.7 |
Completeness [%] | 94.4 | 75.3 |
Redundancy | 1.9 | 1.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.1 | 295.5 | MPEG 550, sodium acetate, sodium citrate, phenol, pH 5.1, VAPOR DIFFUSION, SITTING DROP, temperature 295.5K |