2EC9
Crystal structure analysis of human Factor VIIa , Souluble tissue factor complexed with BCX-3607
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 98 |
Detector technology | IMAGE PLATE |
Collection date | 2004-09-12 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.780, 81.410, 125.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 2.000 |
Rwork | 0.242 |
R-free | 0.28200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dan |
RMSD bond length | 0.006 |
RMSD bond angle | 1.580 |
Data reduction software | d*TREK |
Data scaling software | CrystalClear |
Phasing software | CNX |
Refinement software | CNX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.079 | 0.357 |
Number of reflections | 47808 | |
<I/σ(I)> | 8.9 | 3.2 |
Completeness [%] | 88.0 | |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 14% (w/v)PEG 4K, 0.1M MgCl2, 0.1M ADA buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |