2EB3
Crystal structure of mutated EGFR kinase domain (L858R) in complex with AMPPNP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000 |
Spacegroup name | I 2 3 |
Unit cell lengths | 144.686, 144.686, 144.686 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.170 - 2.840 |
R-factor | 0.19 |
Rwork | 0.190 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gs2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.940 |
High resolution limit [Å] | 2.840 | 2.840 |
Number of reflections | 12050 | |
<I/σ(I)> | 53.8 | 5.833 |
Completeness [%] | 100.0 | 100 |
Redundancy | 29.422 | 29.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.7 | 293 | 0.1M Tris-HCl, 28% PEG 3000, pH 8.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |