2CG5

Structure of aminoadipate-semialdehyde dehydrogenase- phosphopantetheinyl transferase in complex with cytosolic acyl carrier protein and coenzyme A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-02-12
Detector	RIGAKU IMAGE PLATE
Spacegroup name	P 32 2 1
Unit cell lengths	69.361, 69.361, 184.715
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.700
R-factor	0.195
Rwork	0.192
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2byd
RMSD bond length	0.013
RMSD bond angle	1.504
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.700	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.090	0.580
Number of reflections	14864
<I/σ(I)>	17.9	3.4
Completeness [%]	99.9	100
Redundancy	10.4	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.4 M (NH4)H2PO4