2C59
gdp-mannose-3', 5' -epimerase (arabidopsis thaliana), with gdp-alpha-d-mannose and gdp-beta-l-galactose bound in the active site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-11-15 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.808, 83.812, 66.293 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 29.880 - 2.000 |
| R-factor | 0.151 |
| Rwork | 0.147 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c54 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.838 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.500 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.080 | 0.450 |
| Number of reflections | 45789 | |
| <I/σ(I)> | 7.9 | 1.7 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 100 MM BIS-TRIS PH 6.0, 2.2 M AMMONIUM SULPHATE, SITTING DROP VAPOUR DIFFUSION. CRYOPROTECTED WITH 6M SODIUM FORMATE. |
