2BRQ
Crystal structure of the filamin A repeat 21 complexed with the integrin beta7 cytoplasmic tail peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-17 |
| Detector | MARRESEARCH MAR165 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.970, 60.000, 51.570 |
| Unit cell angles | 90.00, 110.17, 90.00 |
Refinement procedure
| Resolution | 37.680 - 2.100 |
| R-factor | 0.197 |
| Rwork | 0.192 |
| R-free | 0.24300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v05 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.465 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.680 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.070 | 0.050 |
| Number of reflections | 13748 | |
| <I/σ(I)> | 17.6 | 5 |
| Completeness [%] | 96.9 | 96.1 |
| Redundancy | 5.2 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 1.26 M SODIUM CITRATE, 0.1 M CITRIC ACID PH 5.5 |
