2BIJ
Crystal structure of the human protein tyrosine phosphatase PTPN5 (STEP, striatum enriched enriched Phosphatase)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-02 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.808, 64.318, 101.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.230 - 2.050 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jln |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.484 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.740 | 2.150 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.090 | 0.370 |
| Number of reflections | 20825 | |
| <I/σ(I)> | 11.14 | 3.05 |
| Completeness [%] | 95.0 | 97 |
| Redundancy | 4.7 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN SOLUTION: 10 MG/ML PTPN5, 50 MM HEPES PH 7.5, 200 MM NACL, 10 MM DTT PRECIPITANT: 25% PEG3350, 0.2 M LI2SO4, 100 MM BIS-TR-S PH 5.5 |
