2BCJ
Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.918, 129.954, 122.764 |
| Unit cell angles | 90.00, 95.81, 90.00 |
Refinement procedure
| Resolution | 46.780 - 3.061 |
| R-factor | 0.236 |
| Rwork | 0.243 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1omw homology model of Galphaq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.917 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.780 | 3.210 |
| High resolution limit [Å] | 3.060 | 3.060 |
| Number of reflections | 37836 | |
| <I/σ(I)> | 13.55 | 1.1 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 9% PEG8000, 1M NaCl, 0.1M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
