2BCJ
Crystal Structure of G Protein-Coupled Receptor Kinase 2 in Complex with Galpha-q and Gbetagamma Subunits
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | G-protein-coupled receptor kinase 2 | polymer | 689 | 79749.9 | 1 | UniProt (P21146) Pfam (PF00615) Pfam (PF00069) Pfam (PF00169) | Bos taurus (Bovine) | Beta-ARK-1, Beta-adrenergic receptor kinase 1 |
| 2 | B (B) | Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 | polymer | 340 | 37311.8 | 1 | UniProt (P62871) Pfam (PF25391) | Bos taurus (Bovine) | Transducin beta chain 1 |
| 3 | C (G) | Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 | polymer | 71 | 7845.1 | 1 | UniProt (P63212) Pfam (PF00631) | Bos taurus (Bovine) | G gamma-I |
| 4 | D (Q) | Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(q) subunit alpha chimera | polymer | 353 | 41298.9 | 1 | UniProt (P10824) UniProt (P21279) Pfam (PF00503) | Rattus norvegicus (Rat) | Adenylate cyclase-inhibiting G alpha protein,Guanine nucleotide-binding protein alpha-q |
| 5 | E (Q) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) PubChem (888) | |||
| 6 | F (Q) | TETRAFLUOROALUMINATE ION | non-polymer | 103.0 | 1 | Chemie (ALF) PubChem (5311325) | |||
| 7 | G (Q) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 8 | H (Q) | water | water | 18.0 | 5 | Chemie (HOH) |
Sequence modifications
A: 1 - 689 (UniProt: P21146)
B: 2 - 340 (UniProt: P62871)
G: 1 - 71 (UniProt: P63212)
Q: 7 - 34 (UniProt: P10824)
Q: 37 - 359 (UniProt: P21279)
| PDB | External Database | Details |
|---|---|---|
| Ala 670 | Ser 670 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ace 1 | - | acetylation |
| PDB | External Database | Details |
|---|---|---|
| Ser 68 | Cys 68 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 35 | - | linker |
| Ser 36 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 166205.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 570.5 | |
| All* | Total formula weight | 166776.1 |
