2BBT
Human deltaF508 NBD1 with two solublizing mutations.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Collection date | 2005-03-11 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 65.405, 65.686, 132.777 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.700 - 2.300 |
R-factor | 0.235 |
Rwork | 0.231 |
R-free | 0.29500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1xmj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.029 |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 63.700 |
High resolution limit [Å] | 2.300 |
Number of reflections | 23718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | 35% PEG4000, 0.2M LiCl, 50mM Tris pH 7.5, 3mM ATP, 5mM MgCl2, 2mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 280K |