2B4Y
Crystal Structure of Human Sirtuin homolog 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9797 |
Spacegroup name | P 1 |
Unit cell lengths | 44.574, 58.756, 104.965 |
Unit cell angles | 93.50, 92.75, 94.98 |
Refinement procedure
Resolution | 40.300 - 1.900 |
R-factor | 0.18527 |
Rwork | 0.183 |
R-free | 0.23604 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ici |
RMSD bond length | 0.019 |
RMSD bond angle | 1.822 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASES |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.300 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 79819 | |
Completeness [%] | 96.1 | 85.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 273 | 18% PEG 4000, 0.1M Hepes, pH 7.5, 10% Isopropanol, VAPOR DIFFUSION, SITTING DROP, temperature 273K |