2AZ9
HIV-1 Protease NL4-3 1X mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-17 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 62.765, 62.765, 81.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 81.700 - 2.500 |
| R-factor | 0.248 |
| Rwork | 0.248 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kzk |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.750 |
| Data reduction software | MOSFLM |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.700 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.011 | 0.403 |
| Number of reflections | 3494 | |
| <I/σ(I)> | 16.2 | 4.6 |
| Completeness [%] | 96.2 | 97.8 |
| Redundancy | 9.4 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.2 | 281.16 | ammonium sulfate, sodium citrate, phosphate buffer, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 281.16K, pH 6.20 |
