2AUS

Crystal structure of the archaeal box H/ACA sRNP Nop10-Cbf5 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9686
Spacegroup name	P 21 21 2
Unit cell lengths	136.430, 136.820, 59.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.100
R-factor	0.225
Rwork	0.224
R-free	0.25200
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.491
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	65534
Completeness [%]	99.8	100
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	potassium phosphate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	potassium phosphate, sodium acetate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.0K