2ARP
Activin A in complex with Fs12 fragment of follistatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.297, 94.502, 44.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.800 - 2.000 |
| R-factor | 0.20189 |
| Rwork | 0.202 |
| R-free | 0.25404 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries: 1s4u 1lr9 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.710 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.427 | |
| Number of reflections | 23201 | |
| <I/σ(I)> | 23.9 | 2.67 |
| Completeness [%] | 98.5 | 98.1 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | PEG-MME 2000, Nickel chloride, Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
