2AR9
Crystal structure of a dimeric caspase-9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-12-01 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 144.698, 78.055, 125.963 |
| Unit cell angles | 90.00, 112.50, 90.00 |
Refinement procedure
| Resolution | 11.000 - 2.800 |
| R-factor | 0.24 |
| Rwork | 0.237 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nw9 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.405 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | |
| Number of reflections | 31920 | |
| Completeness [%] | 99.0 | 98.4 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 290 | PEG5000 monomethylether, MES, tacsimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
