2ANR
Crystal structure (II) of Nova-1 KH1/KH2 domain tandem with 25nt RNA hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.919826 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.453, 37.885, 38.523 |
| Unit cell angles | 90.00, 96.64, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.940 |
| R-factor | 0.23 |
| Rwork | 0.227 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ann |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.682 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.600 | 2.010 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.114 | 0.464 |
| Number of reflections | 16720 | |
| Completeness [%] | 92.5 | 89.6 |
| Redundancy | 6.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | PEG 4000, isopropanol, potassium cloride, magnesium cloride, pH 5.6, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | isopropanol | ||
| 3 | 1 | 1 | potassium chloride | ||
| 4 | 1 | 1 | magnesium chloride | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 4000 | ||
| 7 | 1 | 2 | potassium chloride | ||
| 8 | 1 | 2 | magnesium chloride |
