2ANN
Crystal structure (I) of Nova-1 KH1/KH2 domain tandem with 25 nt RNA hairpin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-11-27 |
| Detector | APS-1 |
| Wavelength(s) | 1.0080 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 155.304, 37.147, 34.825 |
| Unit cell angles | 90.00, 94.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.300 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Nova-2 KH3 domain from PDB ENTRY 1EC6 and idealised 5 bp A-RNA |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.335 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.068 | 0.444 |
| Number of reflections | 9118 | |
| Completeness [%] | 99.7 | 97.9 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 293 | PEG 4000, isopropanole, sodium citrate, potassium chloride, magnesium chloride, pH 5.6, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 10 | 1 | 2 | magnesium chloride | ||
| 2 | 1 | 1 | isopropanole | ||
| 3 | 1 | 1 | sodium citrate | ||
| 4 | 1 | 1 | potassium chloride | ||
| 5 | 1 | 1 | magnesium chloride | ||
| 6 | 1 | 1 | H2O | ||
| 7 | 1 | 2 | PEG 4000 | ||
| 8 | 1 | 2 | sodium citrate | ||
| 9 | 1 | 2 | potassium chloride |
