2AG2
Crystal Structure Analysis of GM2-activator protein complexed with Phosphatidylcholine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2003-08-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.530, 86.190, 120.480 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.23100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1PU5 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.067 | 0.407 |
| Number of reflections | 61655 | |
| <I/σ(I)> | 22.7 | 2.9 |
| Completeness [%] | 99.2 | 93.7 |
| Redundancy | 5.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 278 | Peg 4000, Hepes, isopropanol, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 278K |
