2A1U
Crystal structure of the human ETF E165betaA mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-05 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8123 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.086, 62.869, 174.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.740 - 2.110 |
| R-factor | 0.1826 |
| Rwork | 0.179 |
| R-free | 0.24695 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1efv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.424 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.084 | 0.266 |
| Number of reflections | 28046 | |
| <I/σ(I)> | 14.4 | 3.4 |
| Completeness [%] | 91.0 | 62.2 |
| Redundancy | 4.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG2000MME, 0.1 magnesium sulphate, 0.1M cacodylic acid, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
